Selected Publications

Full list of publications can be found on my Google Scholar profile or including abstracts, represenative figures, and link to full-text PDFs here

The emerging clinical use of targeted fluorescent agents heralds a shift in intraoperative imaging practices that overcome the limitations of human vision. However, in contrast to established radiological methods, no appropriate performance specifications and standards have been established in fluorescence molecular imaging. Moreover, the dependence of fluorescence signals on many experimental parameters and the use of wavelengths ranging from the visible to short-wave infrared (400–1,700 nm) complicate quality control in fluorescence molecular imaging. Here, we discuss the experimental parameters that critically affect fluorescence molecular imaging accuracy, and introduce the concept of high-fidelity fluorescence imaging as a means for ensuring reliable reproduction of fluorescence biodistribution in tissue.
Nature Photonics, Volume 12, pages505–515 (2018),2018
PDF

A long-standing objective in neuroscience has been to image distributed neuronal activity in freely behaving animals. Here we introduce NeuBtracker, a tracking microscope for simultaneous imaging of neuronal activity and behavior of freely swimming fluorescent reporter fish. We showcase the value of NeuBtracker for screening neurostimulants with respect to their combined neuronal and behavioral effects and for determining spontaneous and stimulus-induced spatiotemporal patterns of neuronal activation during naturalistic behavior.
Nat. Methods, vol. 14, no. 11, pp. 1079–1082, 2017,2017
PDF Project Video

Projects

Cryoslicer based Imaging

By adding a widefield, multispectral, reflection and fluorescence imaging system on top of a cryotome, we are able to acquire whole-body 3D anatomical and molecular information as part of the normal histopathology pipeline.

Fast Volumetric Imaging

Combining Camera acquitions with either (1) single plane excitation (SPIM) or (2) detection through microlens arrays (LF)we can achieve real-time 3D information. The SPIM system provided cellular resolution through scanning, while LF is a single snashop volumetric method.

Multispectral Imaging

Probing tissue (and tissue mimmicking phantoms) across a wide range of visible and infrared wavelengths, enable retreaval of biological relevant information(e.g. oxygenation) and engineering optimized imaging methods and systems.

Tracking Microscope

NeuBtracker is a tracking microscope for neurobehavioral interogation of Zebrafish larvae. Based on two camera and 3D scanner (xy-mirrors and tunable lens), the system can provide high resolution calcium recording from the brain of freely swimming fish with minimal pertubations.

Teaching

Courses

The Professors of the Chair of Biological Imaging of the Technical University of Munich, are teaching a variety of pre/post-grad course at both engineering and Medical faculties. I am happy to be entrusted with assisting in some of them including:

  1. Optical Electrophysiology part of the Neuro-Recording Moduletaught to the Elite Master Program in Neuroengineering, including Microscopy Tutorial focusing on Calcium Imaging and fundamentals of Calcium Trace Analysis using Matlab.
  2. Clinical Optical Imaging & Image Analysis (using FIJI) parts, of the post-grad course Preclinical Imaging Class of the Faculty of Medicine.

Student’s Projects Supervision

Motivated students have the chance to work on related projects. I (co-/) supervised a series of PhD-, Master-, and Bachelor-level students as part of their thesis or semester research projects, in a variety of topics including: (1) HDR Fluor. Imaging, (2) Correction of Out-Of-Plane Singal in Episcopic Fluor. Imaging, (3) 3D Printing of medical data, (4) SPIM(Selective Plane Illumination Microscopy), (5&6) Tracking using CNN(Convolutional Neuronal Networks) and on Embedded Systems like NVIDIA Jetson.

Extracurricular activities

During my undergraduate years, I had the chance to devote my free time to several engineering projects with strong social and environmental aspects.

  • In May 2006, the European Social Forum was organised in Athens and the need for a cost-effective and user-friendly interpretation system motivated the formation of AL.I.S team. The team aimed to create an ALternative Intrepration System to allow activists to express themselves in the various social forums and initiatives using an affordable, lightweight and portable interpretation system.

  • Later on, I did an internship at a shipyard assisting the building of the Υδριάδα. Υδριάδα is an offshore and wind/solar-powered desalination plant, build to provide fresh water in remote and off-the-grid islands in Aegean see in environmentally friendly and sustainable way.

  • During the last two years of my studies, I was actively involved at the management of both student organisations of the faculty of Electrical and Computer Engineering (treasurer of IEEE Student Branch, chairman of EESTEC LC Athens ). As EESTEC Local Committee Athens, we organised (fundraising, logistics, e.t.c) both academic (30 participants) and statutory (100+ participants) international events.

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